Year 2016, Volume 1, Issue 2
| Year : | 2016 |
| Volume : | 1 |
| Issue : | 2 |
| Authors : | Olga I. LAZAREVA, Tatiana N. SIVKOVA |
| Title : | EFFECT OF SOMATIC ANISAKIS SIMPLEX EXTRACTTO DEVELOPMENT CHICK EMBRYOS |
| Abstract : | Helminthes have embryotoxic and teratogenic effects to host’s cells and tissues. Investigations were carried out mainly on embryo and fetus in mammals. Blaszkowska, J. (1998); Kadlubowski R. (2000) conducted studies of in chick embryos. Dose-dependent effect is also studied and found that small doses cause embryotoxic effect, while higher doses - teratogenic. Anisakis simplex antigens have also embryotoxic effect has been found. The aim of our research was to examine the effect of A. simplex somatic antigen to the development of chick embryos in its experimental introduction in various ways and at different stages of embryogenesis. For the study chicken embryos were used at different stages of embryogenesis: 7.5, 10.5 and 12.5 days. Methods for introducing antigen chicken embryos: in the allantois cavity, on-chorion allantois membrane and yolk sac at 0.2 ml. The autopsy was performed on embryos 2 and 8 days. The eggs and embryos were weighed and evaluated the embryonic development. As a result of the first experience the greatest change observed by mass, and when administered in the development of antigen in both yolk sac on day 2 (1.68 ± 0.06, unlike control 2.25 ± 0.20), and at 8 days ( 9.97 ± 6.21 in the experiment versus 14.89 ± 0.78 in the control), respectively. In the second experiment eggs were used at 1 and 5 day incubation embryos. The antigen was administered in both cases also in the yolk sac at a dose of 0.2 ml. In the second experiment there was a delay in the development of both cases at autopsy after 2 days in non-incubated eggs missing the development of, unlike the control, the development of which 48 hours corresponded; including 5 daily observed decrease in weight 0.88 ± 0.22, against 1.05 ± 0.05 and 8 days in a similar way. As a result of experiments on the effect of the Anisakis simplex somatic antigen on the development of chick embryos embryotoxic action installed. Thus antigen has the greatest effect on development of early embryos and putting it into the yolk sac. |
| For citation : | Lazareva, O. I., Sivkova, T. N. (2016). Effect of somatic anisakis simplex extract to development chick embryos. AGROFOR International Journal, Volume 1. Issue No. 2. pp. 119-124. DOI:10.7251/AGRENG1602119L |
| Keywords : | Anisakis simplex antigen, chicken embryos, embryotoxic, teratogenic |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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