Year 2026, Volume 11, Issue 1
| Year : | 2026 |
| Volume : | 11 |
| Issue : | 1 |
| Authors : | Akife DALDA-SEKERCI, Fatma BULUT-TOPBAS, Hande Seda ÖZDAL |
| Title : | OPTIMIZATION OF SURFACE STERILIZATION FOR AFRICAN VIOLET (Saintpaulia ionantha) IN TISSUE CULTURE |
| Abstract : | Plant tissue culture studies, conducted under aseptic conditions, are of great importance for the rapid propagation of virus-free healthy plants and for various biotechnological applications. The success of such studies largely depends on the initial step of the culture process: surface sterilization of the explants. This study focused on the sterilization of initial plant material used in tissue culture applications of Saintpaulia ionantha H. Wendl. (African violet). Leaf tissue was used as the explant material. A total of five different sterilization protocols were tested. As a preliminary sterilization step, leaf explants were rinsed under running tap water for 30 minutes. For surface sterilization, explants were treated with sodium hypochlorite (NaClO) solutions at different concentrations (10–30%) and exposure times (10, 15, and 20 minutes). The effectiveness of various combinations using antibacterial liquid soap and Tween 20 was also evaluated. Following sterilization, the leaf explants were cultured on Murashige and Skoog (1962) nutrient medium without plant growth regulators. Initial signs of contamination were observed on the 6th day of culture. The most effective sterilization treatment was obtained with a solution containing 15% sodium hypochlorite and Tween 20, where 96% of the explants remained sterile and viable. These findings demonstrate the effectiveness of this protocol and provide a useful reference for the in vitro propagation of Saintpaulia ionantha. |
| Keywords : | African Violet, Ornamental plants, in vitro. |
ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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