Year 2025, Volume 10, Issue 1
| Year : | 2025 |
| Volume : | 10 |
| Issue : | 1 |
| Authors : | Esra ÖZCAN, Hasan Hüseyin ATAR |
| Title : | OPTIMIZATION OF NUTRIENT MEDIA FOR RAPID AND EFFICIENT MICROPROPAGATION OF BACOPA MONNIERI AND CERATOPHYLLUM DEMERSUM |
| Abstract : | Bacopa monnieri L. Pennell, which has an important medicinal value and Ceratophyllum demersum L. having a great potential in cleaning water contaminated with heavy metals are also an indispensable plants in aquariums. Due to these features, the production of uniform and healthy plants in abundance in these species is important. On the other hand, it is possible to produce thousands of healthy plants with the same genetic structure in a short time with in vitro techniques. In this study, the effects of 12 different basic nutrient media, six different sucrose and seven different agar concentrations were investigated for the rapid and low-cost in vitro production of B. monnieri and C. Demersum species. When shoot formation and rooting rate were evaluated together, it was observed that Schenk ve Hildebrandt (SH) for C. demersum and Orchimax basic nutrient media for B. monnieri gave better rate of micropropagation than other media tested. In different sugar applications, the highest micropropagation results were obtained from the medium containing 20 g/L sucrose in B. monnieri and 50 g/L sucrose in C. demersum. In addition, C. demersum achieved the highest micropropagation in liquid medium without agar, and B. monnieri in medium containing 5 g/L agar. As a result of this study, micropropagation was achieved at high rates in both species without using agar, the most expensive compound of nutrient media, or by using it at low rates. Regenerated plants were transferred to aquariums and achieved 100% adaptation. |
| Keywords : | In vitro, SH medium, Orchimax medium, agar, sucrose. |
ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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