Year 2024, Volume 9, Issue 3
| Year : | 2024 |
| Volume : | 9 |
| Issue : | 3 |
| Authors : | Yupin PHASUK, Julanee NUAMJIT, Thevin VONGPRALUB |
| Title : | NUCLEOTIDE POLYMORPHISMS OF HEAT SHOCK PROTEIN 70-2 GENE (HSP70-2) BETWEEN TROPICAL (THAI NATIVE) AND TEMPERATE (HOLSTEIN FRIESIAN) CATTLES |
| Abstract : | Tropical breed cattle (Bos indicus) have more physiological tolerance to heat than temperate breed cattle (Bos taurus). This phenomenon involves cellular protein production related to various cellular proteins including heat shock protein. The study aimed to compare the nucleotide sequence of the heat shock protein 70-2 gene (HSP70-2) between Thai Native (NT) and Holstein Friesian (HF) cattle by using the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method. DNA was extracted from 70 and 52 samples of NT and HF, respectively. Ten primers (HP1 to HP10), specifically designed for HSP70-2, were related to the data of Bos taurus in GenBank databases (accession number; BTU02892). The primers included three regions of the gene: (1) 5’ flanking region, (2) coding sequence, and (3) 3’ flanking region. The results of 10 primers showed 32 patterns in both NT and HF. Sequence analysis showed polymorphism in HSP70-2 and differentt nucleotide sequences at position 1262 (NT=A/C; HF=G) in HP5 primer, 2347 (NT=T; HF=C) in HP9 primer, and 2515 (NT=G/T; HF=C), 2516 (NT=C/T ; HF=G), and 2557 (NT=G; HF=T) in HP10 primer. The finding suggested that these primers were the best to separate NT from HF, and the different positions were found in HP5 and HP9 primers covering the coding sequence region, while HP10 primer covered the 3’flanking region. Thus, the coding sequence region and 3’flanking may affect the mechanism of HSP70-2 protein translation and HSP70-2 protein post-transcription, respectively. |
| For citation : | Phasuk, Y., Nuamjit, J., Vongpralub, T. (2024). Nucleotide polymorphisms of heat shock protein 70-2 gene (HSP70-2) between tropical (Thai native) and temperate (Holstein friesian) cattles. AGROFOR International Journal, Volume 9. Issue No. 3. pp. 101-109. DOI: 10.7251/AGREN2403101P |
| Keywords : | Tropical breed, Temperate breed, Nucleotide sequence, HSP70-2, Breed difference. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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