Year 2023, Volume 8, Issue 2
| Year : | 2023 |
| Volume : | 8 |
| Issue : | 2 |
| Authors : | Messaouda BELAID M, Nora CHAHBAR, Fatma ACHEUK, Abdelkader OUIDAH, Mohamed Oussama AOMICHE |
| Title : | BACTERIAL ECTOMICROFLORA OF VARROA DESTRUCTOR, ECTOPARASITE OF HONEYBEE, COLLECTED IN THE APIARY OF BOUMERDES (ALGERIA) |
| Abstract : | Varroa destructor Anderson and Trueman (Acari: Varroadae), previously known as Varroa jacobsoni, is an important pest of the honeybee, Apis mellifera L. It has been causing severe damage to populations of this species worldwide in recent years. The aim of this work was the isolation and identification of the bacterial ectomicroflora of the Varroa destructor, an ectoparasite of the bee (Apis mellifera L). Samples of Varroa were collected from the location of Boumerdes (situated in northern Algeria) beehive summer debris. The ectoparasitic honeybee Varroa was disinfected with 70% ethanol and then it was spread in nutrient agar plates. For the isolation and identification of the bacteria, the macroscopic and microscopic characters were done according to Bergey’s manual of systematic Bacteriology. Biochemical characteristics were tested by using API 20E galleries (Biomerieux). The experiments were performed twice. The results of the preliminary study showed that the ectoparasite harbored seven genera of bacteria: Staphylococcus sp (3), Bacillus sp (2) and Pseudomonas sp (2). The colonies of Staphylococcus are Gram positive, mobile, coccoid shaped, aero-anaerobic and with a positive catalase. Bacillus are Gram-staining-positive rods, mobile, endospore forming, aerobes or facultative anaerobic and can produce catalase and oxidase. Pseudomonas bacteria are Gram-negative, oxidase-positive, strict aerobic and non-spore forming. |
| For citation : | Belaid, M. M., Chahbar N., Acheuk F., Ouidah, A., Oussama Aomiche, M. (2023). Bacterial ectomicroflora of Varroa destructor, ectoparasite of honeybee, collected in the apiary of Boumerdes (Algeria). AGROFOR International Journal, Volume 8. Issue No. 2. pp. 24-31. DOI: 10.7251/AGRENG2302024B |
| Keywords : | Apis mellifera L, Varroa destructor, bacterial ectomicroflora, beehive summer debris. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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