Year 2022, Volume 7, Issue 3
| Year : | 2022 |
| Volume : | 7 |
| Issue : | 3 |
| Authors : | Iryna KONDRATSKAYA, Andrei YUKHIMUK, Olga CHIZHIK, Vladimir RESHETNIKOV |
| Title : | DNA MARKERS AS A MEANS OF ASSESSING THE GENETIC DIVERSITY AND IDENTIFICATION OF GRASSES |
| Abstract : | The effectiveness of breeding is largely depends on the presence of the gene variability in the initial breeding material. The genetic variability assessment helps to evaluate the source material during a fundamentally new forms with economically valuable traits creating. In this regard, the assessment of the genetic polymorphism of the cereal grasses genotypes which are of interest both as a source of breeding material and as a variety candidate was carried out by DNA markers. Seventy-three loci were identified for the Festulolium and Lolium L. genotypes using the SCoT marker system, 69 of markers were polymorphic. This marker system revealed a high level of polymorphism in the studied genotypes of Festulolium and Lolium L. – 94.52%. For the genotypes of intergeneric hybrids of the Agropyron L. genus and their parent forms 90 loci were identified – 46 SCoT markers and 44 SRAP markers. From the total pool of markers 73 markers were polymorphic. On average, the level of polymorphism was 81.1%. For the genotypes of the Alopecurus L. genus interspecific hybrids and their parent forms 157 loci were identified – 52 for RAPD-PCR and 105 – for ISSR-PCR. Of the total pool of markers, 104 were polymorphic According to the results of DNA genotyping the genetic passports of cereal grasses were compiled. |
| For citation : | Kondratskaya, I., Yukhimuk, A., Chizhik, O., Reshetnikov, V. (2022). DNA markers as a means of assessing the genetic diversity and identification of grasses. AGROFOR International Journal, Volume 7. Issue No. 3 pp. 112-117. DOI: 10.7251/AGRENG2203112K |
| Keywords : | cereal grasses, genetic passport, genotype, SCoT markers, ISSR, RAPD, SRAP. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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