Year 2022, Volume 7, Issue 1
| Year : | 2022 |
| Volume : | 7 |
| Issue : | 1 |
| Authors : | Muhammad AKRAM, Faheem AFTAB |
| Title : | IN VITRO CHARACTERIZATION OF FIXED OIL CONTENTS FROM CALLUS CULTURES OF JOJOBA (SIMMONDSIA CHINENSIS L.) |
| Abstract : | Objective of the present study was to investigate and determine fixed oil (lipids) contents from in vitro induced calluses of leaf, cotyledon and intermodal explants of jojoba (Simmondsia chinensis L.). The leaf and internodal explants were from micropropagated in vitro plants whereas mature seeds were used for the acquisition of cotyledons. Sterilized explants were cultured on different concentrations (1, 2, 4, 6, 8 or 10 µM) of either 2, 4-Dichlorophenoxyacetic acid (2, 4-D) or α-Naphthaleneacetic acid (NAA) alone or in combination (1+1, 2+1, 4+1, 6+2, 8+2 or 10+2 µM) with 6-Benzylaminopurine (BAP) or Kinetin (Kin) for callus induction. Highly proliferating callus lines (CLs) growing on 8 µM 2, 4 - D (CL-1), 10 µM 2, 4-D (CL-2) and 2, 4-D (10 µM) + 2 µM BAP (CL-3) were selected for the determination of fixed oil contents by distillation method. Highest rate of callus induction (100%) as well as 6.87 gm fresh weight was obtained from leaf explant on CL-3 after 99 days of culture. Amount of fixed oil content (290mg/6gm FW), acid value (0.320), free fatty acid (0.160) and saponification number (91) were highest from calluses of cotyledons on CL-1 as compared to other callus lines CL-2 or CL-3. The present investigation demonstrated an efficient method for determination of the fixed oil contents and related biochemical parameters from calluses of jojoba. |
| For citation : | Akram, M., Aftab, F. (2022). In vitro characterization of fixed oil contents from callus cultures of jojoba (Simmondsia chinensis L.). AGROFOR International Journal, Volume 7. Issue No. 1. pp. 80-89. DOI:10.7251/AGRENG2201080A |
| Keywords : | Callus, fixed oil, free fatty acid, jojoba, liquid wax. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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