Year 2022, Volume 7, Issue 1

Year : 2022
Volume : 7
Issue : 1
Authors : Rita SZABÓ, László MAJOR, József LEHEL, Nadhirah Binti SAIDON, Péter BUDAI
Abstract : The objective of this study was to determine the single and simultaneous toxic effects of chlorpyrifos containing insecticide formulation (Pyrinex 48 EC) and tebuconazole containing fungicide formulation (Mystic 250 EW) on the development of chicken embryos. Amount of 0.1 ml of 1% Pyrinex 48 EC and of 0.4% Mystic 250 EW was alone and concomitantly injected into the air chamber of eggs on the first day prior to incubation. The chicken embryos were examined on day 19 of incubation for the followings: number of embryonic deaths, body, liver and heart weight of the embryos, and type of developmental anomalies. The body, liver and heart weight data were evaluated statistically by One-Way ANOVA, Tukey and Dunnett tests, the embryonic mortality and the developmental abnormalities were analysed by Fisher’s exact test. The combined administration of Pyrinex 48 EC and Mystic 250 EW pesticides on the chick embryo had shown to be embryotoxic to the chick. The rate of mortality and the incidence of developmental anomalies were increased due to the simultaneous application of them. The body and liver weight were significantly reduced. Our teratogenicity study revealed that the combined administration of both chlorpyrifos (Pyrinex 48 EC) and tebuconazole (Mystic 250 EW) pesticides on the chick embryo had shown to be embryotoxic to the chick. They had a slight addition effect on the rate of embryonic mortalities, however, the toxic interaction of both pesticides on developmental anomalies, liver and body weight was not proven.
For citation : Szabó, R., Major, L., Lehel, J., Saidon, N. B., Budai, P. (2022). Teratogenicity testing of chlorpyrifos and tebuconazole in chicken embryos after simultaneous administration. AGROFOR International Journal, Volume 7. Issue No. 1. pp. 40-47. DOI:10.7251/AGRENG2201040S
Keywords : chlorpyrifos, tebuconazole, teratogenicity, chicken embryo, developmental anomalies.
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