Year 2017, Volume 2, Issue 1

Year : 2017
Volume : 2
Issue : 1
   
Authors : Oksana GREBENNIKOVA, Anfisa PALIY, Valentina BRAILKO, Olga MITROFANOVA Valery RABOTYAGOV, Irina ZHDANOVA, Irina MITROFANOVA
Title : ADAPTIVE CAPACITY OF SOME LAVENDER AND LAVANDIN CULTIVARSIN VITRO AND IN SITU
Abstract : Lavandula angustifolia Mill.and (LavandulaxintermediaEmericexLoisel) are promising fragrant plants with medicinal, aromatic and ornamental properties. Since the collection plantations of these crops are very damaged with viral pathogens and there is lack of seed propagation in valuable cultivars 'Belyanka', 'Record' (lavender) and 'Rabat', 'Snezhnyi Bars' (lavandin), were introduced in vitro. Chemotherapy was used for cleaning up. Regenerants were cultured (4-5 months) on MS medium with 0.3 mg L-1 Kinetin, 0.025 mg L 1 NAA and 0.25 mg L-1 GA3 at 25±1°C under 16-h photoperiod. Intact plants were studied during the growing season. In order to reveal plants` biotechnological and genetic capacity some biochemical stress indicators, indexes of photosynthetic activity and water regime were identified. Under the open field cultivation, tested plants were rich in ascorbic acid, phenolic compounds, and redox enzymes (catalase, polyphenol oxidase, superoxide dismutase) were active. Leaf tissue hydration was 56-62%, with greater part of bound water. Photosynthetic activity was reduced only in the samples with visible damages with viral pathogens. In plants cultured in vitro, amount of ascorbic acid and phenolic compounds were lower, so as enzymatic activity and proline concentration were higher than in intact plants. The rate of hydration was high (70-77%), with the same trend of water fractional composition. Photosynthetic activity and vitality index indicated no photoinhibition. It was found out the lavandin cultivars had better capacity for a wide use under various culture conditions.
For citation : Grebennikova, O., Paliy, A., Brailko, V., Mitrofanova, O., Rabotyagov, V., Zhdanova, I., Mitrofanova, I. (2017). Adaptive capacity of some lavender and lavandin cultivars in vitro and in situ. AGROFOR International Journal, Volume 2. Issue No. 1. pp. 91-98. DOI: 10.7251/AGRENG1701091G
Keywords : Lavandula sp, tissue culture, biochemical indicators, photosynthetic activity, water regime.
   
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