Year 2016, Volume 1, Issue 3

Year : 2016
Volume : 1
Issue : 3
Authors : Veronika KUKUČKOVÁ, Nina MORAVČÍKOVÁ, Radovan KASARDA
Abstract : The aim of this study was to assess genetic structure of Slovak Pinzgau population based on polymorphism at molecular markers using statistical methods. Female offspring of 12 most frequently used bulls in Slovak Pinzgau breeding programme were investigated. Pinzgau cattle were found to have a high level of diversity, supported by the number of alleles observed across loci (average 5.31, range 2-11) and by the high within-breed expected heterozygosity (average 0.66, range 0.64-0.73). The state of genetic diversity is satisfying and standard for local populations. Detection of 12 possible subpopulation structures provided us with detailed information of the genetic structure. The Bayesian approach was applied, detecting three, as the most probable number of clusters. The similarity of each subpopulation using microsatellites was confirmed also by high-throughput molecular data. The observed inbreeding (FROH=2.3%) was higher than that expected based on pedigree data (FPED=0.4%) due to the limited number of available generations in pedigree data. One of the most important steps in development of efficient autochthonous breed protection programs is characterization of genetic variability and assessment of the population structure. The chosen set of microsatellites confirmed the suitability in determination of the subpopulations of Pinzgau cattle in Slovakia. The state of genetic diversity at more detailed level was successfully performed using bovineSNP50 BeadChip.
For citation : Kukučková, V., Moravčíková, N., Kasarda, R. (2016). Genomic determination of the most important father lines of Slovak Pinzgau cows. AGROFOR International Journal, Volume 1. Issue No. 3. pp. 110-118. DOI:10.7251/AGRENG1603110K
Keywords : genetic differentiation, microsatellites, Pinzgau cattle, SNP chip, structure
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