Year 2023, Volume 8, Issue 1
| Year : | 2023 |
| Volume : | 8 |
| Issue : | 1 |
| Authors : | Maria RYAZANOVA, Irina ANISIMOVA, Olga VORONOVA |
| Title : | POLLEN FORMATION IN SUNFLOWER HYBRIDS ON THE BASIS OF CMS |
| Abstract : | In externally fertile plants, the quality of the pollen may be different, and it is possible to distinguish the so-called "semi-fertile" forms. This is typical for sunflower F2 hybrid populations from crossing CMS PET1 line and restorer lines. We have characterized the fertility/sterility manifestation in a sample set of 17 F2 and 7 F3 genotypes derived from a cross between the CMS line VIR 116A and a restorer line VIR195. The segregation pattern for fertility/sterility in the original F2 population of 262 genotypes fitted three (fertile): one (sterile) model that supports the hypothesis on controlling the fertility restoration by a single locus (putatively Rf1). Each plant was phenotyped under field conditions and genotyped using SSR marker ORS511 linked the to the Rf1 locus. After flowering, the fertile or sterile plants were registered. The pollen of fertile and chimeric plants was stained with acetocarmine. F1 plants produced appr. 90% of fertile pollen. Among F2 plants examined, five plants were classified as purely fertile, seven as sterile, three as semi sterile, and two as chimeric ones. Among the F3 plants three fertile, three sterile, and one chimeric plant have been noted. The chimeras possessed fertile flowers in the 1st to 3rd cycles of flowering, and further to the center the flowers were sterile. In most cases, fertile and chimeric plants possessed the ORS511 marker whereas sterile plants lacked the marker. The occurrence of the marker in sterile genotypes, and the absence of it in fertile ones can be a consequence of recombination events. |
| For citation : | Ryazanova, M., Anisimova, I., Voronova, O. (2023). Pollen formation in sunflower hybrids on the basis of cytoplasmic male sterility. AGROFOR International Journal, Volume 8. Issue No. 1 pp. 30-37. DOI: 10.7251/AGRENG2301030R |
| Keywords : | pollen fertility, CMS, sunflower, SSR marker ORS511, Rf1 gene. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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