Year 2021, Volume 6, Issue 1
| Year : | 2021 |
| Volume : | 6 |
| Issue : | 1 |
| Authors : | Najwa M. ALHAJJAR, Bayan M. MUZHER |
| Title : | ASSESSMENT OF GENETIC VARIATION AMONG PISTACIA ATLANTICA DESF. REGARDING SEXUAL GENOTYPES (MALE, FEMALE AND HERMAPHRODITIC) USING ISSRS. MARKERS |
| Abstract : | All Pistacia species are dioecious, male and female flowers are born on separated trees. Our recent studies identified new hermaphroditic genotypes of P. atlantica with different structure of racemes and flowers at the south of Syria. Therefore, the current research aimed to assess genetic variation among 11 genotypes (3 female, 5 hermaphroditic, 3 male) across fifteen ISSRs primers in Sweida Research Center (2018-2019). All of the primers were able to detect the polymorphism, which revealed 214 bands, 205 of them were polymorphic (95.79%). The number of bands for each primer ranged from 6 to 33, with an average 14.27 bands for each Primer. Genetic similarity among all studied genotypes ranged from (0.27) between hermaphroditic genotype (PA52) with female genotype (FA3) as well as between MA3 male genotype and FA2 female genotype, while the highest genetic similarity was 0.77 between two hermaphroditic genotypes (PA37and PA52). Cluster analysis grouped all studied genotypes into three main clusters according to their sexual structure; the first cluster contained all of the hermaphroditic genotypes and the second cluster comprised of all male genotypes, while the third cluster included all female genotypes. The results demonstrated the importance and the efficiency of ISSR technique by revealing the genetic variation among P. atlantica genotypes and separating all of them into detached clusters according to their sexual structure. Farther more, some primers were able to detect common bands in each sexual structure which might help to understand the mechanism of sexual inheritance within the studied species. |
| For citation : | Alhajjar, N., M., Muzher, B., M. (2021). Assessment of genetic variation among Pistacia atlantica desf. regarding sexual genotypes (male, female and hermaphroditic) using ISSRS. markers. AGROFOR International Journal, Volume 6. Issue No. 1. pp. 36-44. DOI:10.7251/AGRENG2101036A |
| Keywords : | hermaphroditic genotypes, genetic similarity, P. atlantica, ISSR technique. |
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ISSN 2490-3434 (Printed)
ISSN 2490-3442 (Online)
ISSN 2490-3442 (Online)
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