Year 2021, Volume 6, Issue 1

Year : 2021
Volume : 6
Issue : 1
Authors : Illia POZHYLOV, Halyna SNIHUR, Irena BUDZANIVSKA
Abstract : Raspberry leaf blotch virus (RLBV) is a new member of the Emaravirus genus (Fimoviridae, Bunyavirales). Representatives of this family are characterized by a segmented "-" RNA genome, helical nucleocapsid and enveloped spherical or pleomorphic virions 80-120nm in diameter. Transmission of these viruses is carried out by eriophyid mites. The virus is widely distributed in Europe. In this paper, the phylogenetic relationships between the Ukrainian RLBV isolate and isolates from the other countries from the GenBank database were investigated. Samples of symptomatic raspberry plants were selected for the work. Total RNA was isolated and RT-PCR was performed using primers to the region of the nucleocapsid protein (P3) gene. The resulting amplicon with a length of about 500bp was sequenced. Sequences were analyzed using BLAST and MEGA7 programs. As a result of BLAST analysis, it was shown that the Ukrainian isolate of RLBV has a high similarity to some Finnish, British, Serbian and Slovak isolates (93-99% similarity). Nevertheless, the dendrogram constructed in MEGA7 did not distribute these isolates in a separate cluster. Interestingly, sequences of isolates from Finland, Britain, and the Balkans were segregated into different parts of the phylogenetic tree. This pattern can be explained by the low divergence of the virus population or small number of isolates in the database.
For citation : Pozhylov, I., Snihur,H., Budzanivska, I. (2021). Phylogenetic analysis of Ukrainian isolate of raspberry leaf blotch virus. AGROFOR International Journal, Volume 6. Issue No. 1. pp. 19-25. DOI:10.7251/AGRENG2101019P
Keywords : Raspberry leaf blotch emaravirus, raspberry, Ukraine
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