Year 2016, Volume 1, Issue 1

Year : 2016
Volume : 1
Issue : 1
Authors : Аlexey LUKIN, Alexander YEPRINTSEV, Dmitry FEDORIN, Olga MARAEVA, Sergey SELYAVKIN
Abstract : At present, there are microbiological Bacillus-based products used to preserve valuable micro biota and improving the level of biological soil productivity as well as sustaining the local environment. The research focuses on discovering how some particular biological products and autochthonous microorganisms influence the yield capacity of barley grown in the Black-Earth region of Russia. The research objectives included a search for autochthonous strains of microorganisms that improve resistance to diseases, estimation of how biological products contribute to the quality of barley seeds, biological products effect on the spread and disease resistance and estimation of how biological products enhance the yield capacity of barley. The paper describes the results of identification of an autochthonous Bacillus strains. PCR diagnostic methods were used to confirm the strain specific origin of two sample cells extracted from soil (S1 and S2). The study involved the analysis of micro biota of leached chernozem, which revealed the autochthonous strain of Bacillus S1 having a germicidal effect. The S1 strain revealed Bacillus subtilis and Bacillus сеreus, while S2 revealed only Bacillus subtilis, as detected by the method of molecular diagnostics based on using species-specific primers. Biological treatment of the seeds improved their sowing qualities, namely, germination readiness and germination capacity. In addition, it was found out that such treatment improves the resistance to disease affection and spread. Bacillus S1, in particular, reduces the disease affection by 16,5 % and the disease spread 3,5 as much. Finally, the experiment demonstrated that biological treatment can contribute to sustaining healthy environment for the plants and thus increase their yield capacity.
For citation : Lukin, A., Yeprintsev, A., Fedorin, D., Maraeva, O., Selyavkin, S. (2016). Ecologically based disease management techniques in barley cultivation in the Central Black soil region. AGROFOR International Journal, Volume 1. Issue No. 1. pp. 41-48. DOI:10.7251/AGRENG1601041L
Keywords : identification of Bacillus, the polymerase chain reaction (PCR), sowing quality of seeds, barley disease control
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