Year 2019, Volume 4, Issue 3

Year : 2019
Volume : 4
Issue : 3
   
Authors : Hacer Handan ALTINOK, Muhammed Bahattin TANYOLAÇ, Duygu ATEŞ, Canan CAN, Hilal ÖZKILINÇ
Title : MOLECULAR PHYLOGENY OF FUSARIUM OXYSPORUM SPECIES COMPLEX ISOLATED FROM EGGPLANT AND PEPPER IN TURKEY
Abstract : Members of Fusarium oxysporum species complex (FOSC) are economically most important plant pathogenic fungi found in a large number of plants in different families, while individual strains have strong specificities for particular hosts. Fusarium wilt caused by F. oxysporum f. sp. melongenae (Fomg) and F. oxysporum f. sp. capsici (Foc) is a worldwide soil-borne disease that causes yield losses in eggplant and pepper (Solanaceae) growing regions of Turkey. The intra-species or inter-species genetic diversity and phylogenetic relationships in Fomg and Foc isolates obtained from Turkey were investigated by utilizing the sequence data obtained through the designed primers belonging to three protein coding gene regions; beta-tubulin (BT), calmodulin (CAL) and chitin synthase (CHS). Phylogenetic analyses were carried out with BT, CAL and CHS gene regions with a selected subset of Fomg and Foc, along with other non-host F. oxysporum and outgroup isolates. The clustering trees successfully separated the Fomg and Foc from the outgroup Fusarium isolates. On the other hand, sequence data for BT, CAL and CHS gene regions displayed limited variation among Fomg and Foc isolates and found inefficient to distinguish reliably among several F. oxysporum forma specialis (f. sp.) groups. The sequence variation on these ‘housekeeping gene’ regions in the core genome was not considered adequate to differentiate FOSC populations.
Keywords : Fusarium wilt, phylogenetic analysis, DNA sequence
   

 

r