Year 2019, Volume 4, Issue 1

Year : 2019
Volume : 4
Issue : 1
Authors : Zeynep SEMEN, Vedat KARAKAŞ, Tuncay ÇÖKÜLGEN, İlker ÜNAL, Onur YILMAZ
Abstract : The present study was conducted in order to reveal the genetic diversity and bottleneck in Anatolian Black Cattle (Bos Taurus). Animal material of the study consisted of 75 cattle raised in International Center for Livestock Research and Training. The bottleneck in the cattle breed studied was checked with 10 microsatellites markers, amplified in a multiplex polymerase chain reaction (PCR) were used according to recommendation of FAO (2011). A total of 116 alleles was observed from microsatellites studied. Overall value belongs to average number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (Ho), expected heterozygosity (He), the polymorphic information content (PIC), average heterozygosity (Ĥ), and FIS, known as the inbreeding coefficient, were 11.60, 5.35, 0.80, 0.78, 0.80 and 0.012, respectively. All microsatellite markers except INRA23 and ETH3 deviated from Hardy Weinberg equilibrium (HWE). Bottleneck was analyzed with Bottleneck software according to three different mutation models including the infinite allele model (IAM), two-phase mutation model (TPM) and stepwise mutation model (SMM). It can be said that there is not any ultimate risk in terms of bottleneck considering L–shaped curve showing normal distribution obtained from the analysis.
For citation : Semen, Z., Karakaş, V., Çökülgen, T., Ünal,I., Yilmaz, O. (2019). Bottleneck analysis of Anatolian black cattle (Bos taurus) using microsatellite markers. AGROFOR International Journal, Volume 4. Issue No. 1. pp. 161-168. DOI: 10.7251/AGRENG1901161S
Keywords : Bottleneck, microsatellite, Anatolian Black Cattle
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