Year 2019, Volume 4, Issue 1

Year : 2019
Volume : 4
Issue : 1
Authors : Penka MONEVA, Ivan YANCHEV, Marina DYAVOLOVA, Dimitar GUDEV
Abstract : The object of the present study was to investigate small and large lymphocytes trafficking in sheep with low and high hematocrit values following shearing, exposure to moderate altitude and transport to low altitude. Twenty out of 101 Ile de France ewes (1-7 years old) were used in the present experiment. All ewes of the flock were artificially inseminated in May 2015 following estrus synchronization. The animals were allocated into two groups following threefold measurements of hematocrit in all ewes as follows: I- low hematocrit group (n=10) and II - high hematocrit group (n=10). The ewes were transported to the Petrohan Pass (1440 m above sea level) in June 2015 immediately after shearing, conducted at the experimental farm of the Institute of Animal Science, Kostinbrod (500 m above sea level). Blood samples were collected before shearing, immediately after shearing, 3 h after shearing, at 14 d following exposure to moderate altitude, immediately after transport to low altitude and following 7d of stay at low altitude. All leukocyte subpopulations were counted microscopically. In the current study we presented the percentage of lymphocytes only, including small and large (reactive) lymphocytes. High and low hematocrit ewes had different percentage of small lymphocytes when exposed to various acute and chronic stress stimuli. There were significant differences in the percentage of large (reactive) lymphocytes between low and high hematocrit ewes following blood collection and immediately after shearing. The observed difference in small lymphocyte dynamics among the groups in response to different stress stimuli was attributed to hematocrit related differences in the time course and magnitude of lymphocyte distribution at early and late phases of stress. The results were interpreted to mean that the differences in lymphocyte trafficking between the two groups of sheep in response to stress were related to possible difference in the share of aerobic and glycolytic pathways for energy supply.
For citation : Moneva, P., Yanchev, I., Dyavolova, M., Gudev, D. (2019). Lymphocyte trafficking following acute stress and altitude hypoxia in low and high hematocrit sheep. AGROFOR International Journal, Volume 4. Issue No. 1. pp. 73-81. DOI: 10.7251/AGRENG1901073M
Keywords : small lymphocytes, large lymphocytes, hematocrit, sheep, stress
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