Year 2018, Volume 3, Issue 3

Year : 2018
Volume : 3
Issue : 3
   
Authors : Elie KHOURY, Tony Kevork SAJYAN, Peter ZHELEV, Youssef Najib SASSINE
Title : INVESTIGATING THE EFFECT OF SUBSTRATE, MYCORRHIZAL APPLICATION AND BULB SEPARATION ON THE GROWTH OF THE WILD ORCHID ANACAMPTIS PYRAMIDALIS
Abstract : Pyramidal orchid (Anacamptis pyramidalis) is a wild terrestrial orchid widely found in Lebanon and the Mediterranean zone. Random collection and trade of the orchid for medicinal and edible use (salep) subjected it to a risk of extinction. Consequently, the current work aimed to propagate this orchid in vivo. Orchid bulbs were transplanted from the wild into four different soil substrates (Pinebark, Pinebark-Peat (1/1), Peat-Sand (1/1) and control: soil from the collection site) under controlled conditions of temperature and humidity. The effect of mycorrhizal application (MY: Yes) was tested in the different substrates except in control and orchid bulbs (son bulb and mother bulb) were planted combined (SB+MB) or after separation of the mother bulb (SB). Same treatments were repeated over two consecutive years. Results showed an earlier emergence of son bulbs (SB) grown in control substrate. Moreover, there was a significant difference in plant growth with superiority for (SB) compared to (SB+MB) regarding plant length and elongation of first leaf except bulb dimensions (length and width) that were higher in (SB+MB). Mycorrhizal application enhanced the overall growth of plants and its effect was the most obvious in the substrate Peat-Sand (1/1). In general, the best growth of the orchid was observed at the level (SB)/(MY:Yes)/Peat-Sand (1/1). Results of the second experimental year confirmed those of the first year with an improvement of the rate of emergence by 13%. The in vivo propagation method was beneficial on improving the growth of A. pyramidalis ex situ and it could be adopted as an initiative for wild orchid conservation.
For citation : Khoury, E., Sajyan, T.K., Zhelev, P., Sassine, Y. N. (2018). Investigating the effect of substrate, mycorrhizal application and bulb separation on the growth of the wild orchid Anacamptis pyramidalis. AGROFOR International Journal, Volume 3. Issue No. 3. pp. 5-13. DOI: 10.7251/AGRENG1803005K
Keywords : Anacamptis pyramidalis, conservation, substrate, mycorrhiza, bulbs
   
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