Year 2018, Volume 3, Issue 2

Year : 2018
Volume : 3
Issue : 2
Authors : Desimir KNEŽEVIĆ, Aleksandra Yu. Novoselskaya DRAGOVIČ, Aleksandr KUDRYAVCEV, Danijela KONDIĆ, Gordana BRANKOVIĆ, Sretenka SRDIĆ, Veselinka ZEČEVIĆ, Tanja MIJATOVIĆ
Abstract : High molecular weight glutenin subunits (HMW-GS) proteins deposited in endosperm of wheat seed which have significant impact on bread quality. The HMW-GS encoded by genes located at the long arm of chromosomes 1A, 1B, 1D. The aim of this work was study allele polymorphysms at Glu-A1, Glu-B1 and Glu-D1 locus and loaf volume, grain protein content, sedimentation volume of eight wheat genotypes (G-3130, G-35183, G-3501, G3512, G-3574, G-3027, G-3075, G-3097) harvested in two years with various weather condition. For each genotypes, flour used for extraction of glutenin which separated by method of electrophoresis on SDS gel (11.8%). Electrophoregrams used for determining Glu-1 alleles. Technological quality parameters analyzed by standard laboratory methods. The three alleles alleles (a, b, c) at the Glu-A1, three alleles (b, c, d) at the Glu-B1 and 2 alleles (a, d) at the Glu-D1 were identified. The highest protein sedimentation volume had wheat genotype G-3075 in the both years (54.0ml; 58.0ml) while the lowest sedimentation volume had G-3512 (34.0ml; 36.0ml). Grain protein content (GPC) was the highest in G-3075 in both years (14.20%; 15.40%) while the lowest GPC had G-3097 (11.60%) in first and G-3512 (12.60%) in the second year. Loaf volume was the highest in G-3075 in both year (520ml; 540ml) while the lowest was in G-3512 (400ml) in both years of experiment. The estimated quality traits varied depending on genotype and year. The better quality, in average, had the wheat genotypes which carried Glu-D1d allele.
For citation : Knežević, D., Novoselskaya Dragovič, A. Y., Kudryavcev, A., Kondić, D., Branković, G., Srdić, S., Zečević, V. (2018). Allelic composition of HMW-glutenin protein and their relationship with quality of wheat. AGROFOR International Journal, Volume 3. Issue No. 2. pp. 14-21. DOI: 10.7251/AGRENG1802014K
Keywords : wheat, glutenin, Glu-1 allele, quality, polymorphism
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