Year 2018, Volume 3, Issue 1

Year : 2018
Volume : 3
Issue : 1
Authors : Eva TVRDÁ, Michal ĎURAČKA, Attila KÁNTOR, Marek HALENÁR, Lukáš HLEBA
Abstract : The aim of this study was to assess the potential efficiency of selected biologically active substances on the motility behaviour of rabbit spermatozoa subjected to in vitro induced A. baumannii contamination. The semen samples used for A. baumannii detection were collected from 10 New Zealand white male rabbits and the presence of the bacterium was confirmed using MALDI-TOF Mass Spectrometry. For the in vitro experiments rabbit spermatozoa were re-suspended in PBS, containing mineral supplements, BSA and glucose in the presence of 3x105 CFU A. baumannii and diverse concentrations of selected biomolecules (resveratrol - RES, quercetin - QUE, curcumin - CUR, epicatechin - EPI, isoquercitrin - ISO). The sperm motility was assessed using the computer-aided sperm analysis at 0h, 2h, 4h and 6h. A. baumannii significantly decreased the sperm motility (P<0.001) at Time 2h and maintained this negative impact throughout the in vitro culture. Meanwhile, the motility at Time 2h was significantly higher in the samples subjected to A. baumannii together with 10 μmol/L RES (P<0.01); 5, 10 and 50 μmol/L QUE (P<0.001); 1 μmol/L CUR (P<0.05); 10, 50 and 100 μmol/L EPI (P<0.01) as well as 50 μmol/L (P<0.05) and 100 μmol/L ISO (P<0.001) in comparison to the control exposed to the bacterium exclusively. After 4h, the motility remained significantly higher in the groups co-treated with the inoculum and 10 μmol/L RES (P<0.05), 50 μmol/L QUE (P<0.05) as well as 50 μmol/L EPI (P<0.05) when compared to the positive control. Nevertheless, none of the biomolecules was effective against the rapid decline of sperm motility caused by A. baumannii during later stages of the experiment (Time 6h). Based on these results, one can conclude that RES, QUE and EPI exhibit antibacterial properties providing a selective advantage to spermatozoa in the presence of A. baumannii, particularly during short-term rabbit semen handling.
For citation : Tvrdá, E., Ďuračka, M., Kántor, A., Halenár, M., Hleba, L. (2018). In vitro effects of Acinetobacter baumannii and selected natural biomolecules on rabbit spermatozoa motility. AGROFOR International Journal, Volume 3. Issue No. 1. pp. 54-63. DOI: 10.7251/AGRENG1801054T
Keywords : Acinetobacter baumannii, spermatozoa, biomolecules, contamination, motility
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