Year 2017, Volume 2, Issue 1

Year : 2017
Volume : 2
Issue : 1
Authors : Nurper GUZ, Aslı DAĞERİ
Abstract : The Egyptian cotton leaf worm, Spodoptera littoralis (Boisd.) is a well-known as one of the most destructive agricultural lepidopterous pests. It is a true generalist species with a promiscuous feeding strategy which enables it to attack numerous economically important crops all year round including vegetables, ornamental plants, and cotton. Recently, chemical control has been commonly used to suppress populations of S. littoralis; however, a very large number of insecticides have ledto the emergence ofresistance. An extensive use of insecticides also has other side effects, including the elimination of non-targeted organisms, environmental damage and harm to human health. Genome-wide high-throughput technologies help developing resistance management strategies, especially identifying genetic mechanisms of resistance. The aim of the present study was to produce a de novo transcriptome for S. littoralis as a resource for current and future studies of this pest species by using next-generation sequencing. This resource was then used as a reference for identifying genes by encoding the target sites of insecticides currently in use for Egyptian cotton leaf worm control. To achieve this, a cDNA library was sequenced using 454 FLX Titanium Sequencing on the Roche platform which revealed good coverage of genes encoding insecticide target sites and detoxification enzymes using a manual annotation. Annotations of assembled sequences were carried out by BLASTx against NCBI non-redundant protein sequence databases using the software Blast2GO. The genesencoding enzymesinvolvedin insecticide detoxification such as Acetylcholinesterase, Cytochrome p450, Glutathione S-transferase were characterized. Furthermore, a phylogenetic analyses based on three protein sequences were generated in order to give evolutionary insights into insecticide resistance gene families of S. littoralis.
For citation : Guz, N., Dağeri, A. (2017). Sequence analysis of insecticide resistance and detoxification related genes in Spodoptera littoralis (Lepidoptera: Noctuidae). AGROFOR International Journal, Volume 2. Issue No. 1. pp. 144-153. DOI: 10.7251/AGRENG1701144G
Keywords : Spodoptera littoralis, transcriptome, insecticide resistance, detoxification
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